6ha sequence (Addgene inc)
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6ha sequence
6ha Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/6ha+sequence/pHyg-AID*-6HA+(Plasmid+%2399520)/pm34695378-108-196-200
Average 92 stars, based on 2 article reviews
6ha Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/6ha+sequence/pHyg-AID*-6HA+(Plasmid+%2399520)/pm34695378-108-196-200
Average 92 stars, based on 2 article reviews
6ha sequence - by Bioz Stars,
2026-09
92/100 stars
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Sequencing:Article Title: Vacuolar Localization via the N-terminal Domain of Sch9 is Required for TORC1-dependent Phosphorylation and Downstream Signal Transduction. Article Snippet: To generate pYTK111 (SCH9-part-4a), the SCH9 sequence was amplified from the yeast genome in 4 fragments to remove BsaI and BsmBI restriction sites via introduction of synonymous mutations (“domestication”), using the following primers: SCH9-YTK-4a-for and SCH9-A-rev, SCH9-B-for and SCH9-B-rev, SCH9-C-for and SCH9-C-rev, SCH9-D-for and SCH9-YTK-4a-rev. pYTK123 (SCH9-part-3) was generated by amplification of SCH9 from pYTK111 using primers SCH9YTK-3-for and SCH9-YTK-3-rev. pYTK136 (SCH9NT-part-3a) was generated by amplification of SCH9aa1-182 from pYTK111 using primers SCH9-YTK-3-for and SCH9-NT-3a-rev. pYTK137 (SCH9NT-part-3b) was generated by amplification of SCH9aa1182 from pYTK111 using primers SCH9-NT-3b-for and SCH9NT-3b-rev. pYTK138 (SCH9CT-part-3b) was generated by amplification of SCH9aa400-824 from pYTK111 using primers SCH9-CT-3b-for and SCH9-YTK-3-rev. pYTK139 (SCH9CTpart-4a) was generated by amplification of SCH9aa400-824 from pYTK111 using primers SCH9-CT-4a-for and SCH9-CT-4a-rev. pYTK189 (sch9DNT-part-3) was generated by amplification of SCH9aa183-824 from pYTK111 using primers SCH9-C2-3-for and SCH9-YTK-3-rev. pYTK205 (sch9DNT-part-3b) was generated by amplification of SCH9aa183-824 from pYTK111 using primers SCH9-C2-3b-for and SCH9-YTK-3-rev. pYTK215 (sch9DNTpart-4a) was generated by amplification of SCH9aa183-824 from pYTK111 using primers SCH9-C2-4-for and SCH9-CT-4a-rev. pYTK109 (pSCH9-part-2) was generated by amplification of 535 bps upstream of the SCH9 ORF from the yeast genome using primers SCH9p-YTK-2-for and SCH9p-YTK-2-rev. pYTK126 (6HA-part-4a) was generated by amplification of the 6HA sequence from Amplification:Article Title: Vacuolar Localization via the N-terminal Domain of Sch9 is Required for TORC1-dependent Phosphorylation and Downstream Signal Transduction. Article Snippet: To generate pYTK111 (SCH9-part-4a), the SCH9 sequence was amplified from the yeast genome in 4 fragments to remove BsaI and BsmBI restriction sites via introduction of synonymous mutations (“domestication”), using the following primers: SCH9-YTK-4a-for and SCH9-A-rev, SCH9-B-for and SCH9-B-rev, SCH9-C-for and SCH9-C-rev, SCH9-D-for and SCH9-YTK-4a-rev. pYTK123 (SCH9-part-3) was generated by amplification of SCH9 from pYTK111 using primers SCH9YTK-3-for and SCH9-YTK-3-rev. pYTK136 (SCH9NT-part-3a) was generated by amplification of SCH9aa1-182 from pYTK111 using primers SCH9-YTK-3-for and SCH9-NT-3a-rev. pYTK137 (SCH9NT-part-3b) was generated by amplification of SCH9aa1182 from pYTK111 using primers SCH9-NT-3b-for and SCH9NT-3b-rev. pYTK138 (SCH9CT-part-3b) was generated by amplification of SCH9aa400-824 from pYTK111 using primers SCH9-CT-3b-for and SCH9-YTK-3-rev. pYTK139 (SCH9CTpart-4a) was generated by amplification of SCH9aa400-824 from pYTK111 using primers SCH9-CT-4a-for and SCH9-CT-4a-rev. pYTK189 (sch9DNT-part-3) was generated by amplification of SCH9aa183-824 from pYTK111 using primers SCH9-C2-3-for and SCH9-YTK-3-rev. pYTK205 (sch9DNT-part-3b) was generated by amplification of SCH9aa183-824 from pYTK111 using primers SCH9-C2-3b-for and SCH9-YTK-3-rev. pYTK215 (sch9DNTpart-4a) was generated by amplification of SCH9aa183-824 from pYTK111 using primers SCH9-C2-4-for and SCH9-CT-4a-rev. pYTK109 (pSCH9-part-2) was generated by amplification of 535 bps upstream of the SCH9 ORF from the yeast genome using primers SCH9p-YTK-2-for and SCH9p-YTK-2-rev. pYTK126 (6HA-part-4a) was generated by amplification of the 6HA sequence from Generated:Article Title: Vacuolar Localization via the N-terminal Domain of Sch9 is Required for TORC1-dependent Phosphorylation and Downstream Signal Transduction. Article Snippet: To generate pYTK111 (SCH9-part-4a), the SCH9 sequence was amplified from the yeast genome in 4 fragments to remove BsaI and BsmBI restriction sites via introduction of synonymous mutations (“domestication”), using the following primers: SCH9-YTK-4a-for and SCH9-A-rev, SCH9-B-for and SCH9-B-rev, SCH9-C-for and SCH9-C-rev, SCH9-D-for and SCH9-YTK-4a-rev. pYTK123 (SCH9-part-3) was generated by amplification of SCH9 from pYTK111 using primers SCH9YTK-3-for and SCH9-YTK-3-rev. pYTK136 (SCH9NT-part-3a) was generated by amplification of SCH9aa1-182 from pYTK111 using primers SCH9-YTK-3-for and SCH9-NT-3a-rev. pYTK137 (SCH9NT-part-3b) was generated by amplification of SCH9aa1182 from pYTK111 using primers SCH9-NT-3b-for and SCH9NT-3b-rev. pYTK138 (SCH9CT-part-3b) was generated by amplification of SCH9aa400-824 from pYTK111 using primers SCH9-CT-3b-for and SCH9-YTK-3-rev. pYTK139 (SCH9CTpart-4a) was generated by amplification of SCH9aa400-824 from pYTK111 using primers SCH9-CT-4a-for and SCH9-CT-4a-rev. pYTK189 (sch9DNT-part-3) was generated by amplification of SCH9aa183-824 from pYTK111 using primers SCH9-C2-3-for and SCH9-YTK-3-rev. pYTK205 (sch9DNT-part-3b) was generated by amplification of SCH9aa183-824 from pYTK111 using primers SCH9-C2-3b-for and SCH9-YTK-3-rev. pYTK215 (sch9DNTpart-4a) was generated by amplification of SCH9aa183-824 from pYTK111 using primers SCH9-C2-4-for and SCH9-CT-4a-rev. pYTK109 (pSCH9-part-2) was generated by amplification of 535 bps upstream of the SCH9 ORF from the yeast genome using primers SCH9p-YTK-2-for and SCH9p-YTK-2-rev. pYTK126 (6HA-part-4a) was generated by amplification of the 6HA sequence from |